Quantitative Human CA12 (Glycosylated) Sandwich ELISA Kit

The quantitative human CA12 (glycosylated) sandwich ELISA kit is designed to detect human carbonic anhydrase 12 (CA12) levels. CA12 is a membrane-associated enzyme that catalyzes the reversible hydration of carbon dioxide, playing a crucial role in pH regulation and ion transport in various tissues. It is expressed in several organs and is notably overexpressed in certain cancers, where it contributes to tumor growth and progression. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 7.101 pg/mL, which can accurately detect low concentrations of CA12 in the sample.

CA12

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

7.101 pg/mL

Quantitative sandwich ELISA

20 pg/mL-800 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Carbonic anhydrase 12

CA12; Carbonic anhydrase 12; CA-XII; CAXII; Carbonate dehydratase XII

28, 80, 162

The quantitative human CA12 (glycosylated) sandwich ELISA kit is utilized in research to investigate the expression patterns of CA12, particularly in cancer studies where it may serve as a potential biomarker for diagnosis or prognosis. By accurately quantifying CA12 levels, researchers can explore its role in disease mechanisms, assess its involvement in tumor development, and evaluate the effectiveness of therapeutic interventions targeting this enzyme.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.