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Quantitative Human TCN1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX281

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Specifications
Product Description
The quantitative human TCN1 (glycosylated) sandwich ELISA kit is designed to detect human transcobalamin 1 (TCN1) levels. TCN1 is a protein that binds to and transports vitamin B12. This protein is found in blood and other bodily fluids, which plays a role in vitamin B12 metabolism and delivery to tissues. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 6.025 pg/mL, which can accurately detect low concentrations of TCN1 in the sample.
Target
TCN1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.025 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Transcobalamin 1
Alternate Names
TCN1; Transcobalamin 1; TCI; TC1
Uniprot No.
N-Glycosylation Site
160, 216, 316, 337, 343, 349, 354, 369
Application Notes
Application
The quantitative human TCN1 (glycosylated) sandwich ELISA kit provides a tool for quantitatively measuring glycosylated TCN1 levels in biological samples. It can be used in studies examining vitamin B12 deficiency, anemia, and neurological disorders. The kit is applicable in hematology research.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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