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Size | Qty | Add To Basket |
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500 mL | ||
1 L |
Product Description | Ham's F12 media for SILAC is a specialized cell culture medium designed specifically for stable isotope labeling by amino acids in cell culture (SILAC) experiments. This medium is formulated without specific essential amino acids, allowing researchers to incorporate isotopically labeled versions of these amino acids into proteins during cell growth. By providing a controlled environment for the incorporation of heavy isotopes, Ham's F12 media for SILAC enables precise quantification of protein expression and dynamics using mass spectrometry. This makes it an invaluable tool for proteomics studies aimed at understanding protein function, interactions, and cellular pathways. Compatible with a wide range of cell types, this medium ensures robust cell growth and viability while facilitating accurate comparative analyses of protein samples. |
Synonyms | Ham's F-12 SILAC medium; Ham's F12 for stable isotope labeling; Ham's F12 cell culture medium for SILAC |
Form | Liquid |
Storage | Store at 2-8°C for long term storage. |
Application | Ham's F12 media for SILAC is a specialized cell culture medium formulated to facilitate stable isotope labeling by amino acids in cell culture (SILAC) experiments, particularly for cell lines that are optimally maintained in Ham's F12-based formulations, such as certain epithelial cells and specialized cell types requiring specific nutrient compositions. This modified Ham's F12 medium is essential for studying cellular responses in these specialized cells, including investigations into cellular differentiation, signaling pathways, and the effects of various stimuli, providing valuable insights into proteomic dynamics within these specific biological contexts. |