L-Arginine-HCl for SILAC

L-Arginine-HCl for SILAC is a standard, unlabeled form of the essential amino acid L-arginine, used as a control or "light" counterpart in stable isotope labeling by amino acids in cell culture (SILAC) experiments. While SILAC typically involves comparing cells grown in media containing isotopically labeled amino acids, a baseline is needed.

1119-34-2

1119-34-2; MFCD00064550; (S)-2-Amino-5-guanidinopentanoic acid hydrochloride; L-ARGININE HCL; L-Arginine hydrochloride; L-(+)-Arginine monohydrochloride; EC 214-275-1; Arginine HCl

C₆H₁₅ClN₄O₂

MFCD00064550

210.66

InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1

KWTQSFXGGICVPE-WCCKRBBISA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

L-Arginine-HCl for SILAC is used to incorporate into the proteins of cells grown in standard media, establishing the "light" proteome. This light proteome serves as the reference against which the "heavy" (labeled) proteome from cells grown in media containing stable isotope-labeled arginine is compared using mass spectrometry. This comparison enables researchers to quantify relative changes in protein abundance between different experimental conditions, making L-arginine-HCl for SILAC essential for setting up the quantitative foundation in SILAC-based proteomics studies of protein expression, post-translational modifications, and protein-protein interactions.