DMEM F12 for SILAC

DMEM F12 for SILAC is a specialized cell culture medium formulated to support stable isotope labeling by amino acids in cell culture (SILAC) experiments. This medium combines the nutrient-rich properties of Dulbecco's Modified Eagle Medium (DMEM) with F12, providing an optimal environment for the growth and maintenance of a wide variety of cell lines. Crucially, DMEM F12 for SILAC is devoid of specific amino acids (typically L-lysine and L-arginine), which allows researchers to supplement the medium with isotopically labeled versions of these amino acids. As cells incorporate these labeled amino acids into newly synthesized proteins during culture, it enables precise quantitative proteomic analysis using mass spectrometry. This facilitates the study of protein expression, post-translational modifications, and protein interactions with high sensitivity and accuracy.

DMEM/F12 SILAC medium; DMEM-F12 isotope labeling media; SILAC-compatible DMEM/F12; Dulbecco's modified eagle medium/F12 for SILAC; DMEM/F12 cell culture media for SILAC

Liquid

Store at 2-8°C for long term storage.

DMEM F12 medium for SILAC is a specialized cell culture medium formulated to facilitate stable isotope labeling by amino acids in cell culture (SILAC) experiments. By utilizing this specialized medium, researchers can precisely compare protein expression levels across diverse experimental conditions.