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Quantitative Human ADAM15 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX567

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Specifications
Product Description
The quantitative human ADAM15 (glycosylated) sandwich ELISA kit is designed to detect human a disintegrin and metalloprotease 15 (ADAM15) levels. ADAM15 is a transmembrane protein, which is a metalloproteinase enzyme. ADAM15 is involved in cell adhesion, cell signaling, and proteolysis of extracellular matrix proteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.069 ng/mL, which can accurately detect low concentrations of ADAM15 in the sample.
Target
ADAM15
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.069 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
A disintegrin and metalloprotease 15
Alternate Names
ADAM15; A disintegrin and metalloprotease 15; MDC15; Metargidin; Metalloprotease RGD disintegrin protein
Uniprot No.
N-Glycosylation Site
237, 389, 392, 606, 611
Application Notes
Application
The quantitative human ADAM15 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and matrix biology, particularly in studies of cell adhesion. Researchers can measure ADAM15 levels in cells and tissues. Applications include studies of cancer, inflammation, and wound healing.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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