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Size | Qty | Add To Basket |
---|---|---|
500 mL | ||
1 L |
Product Description | DMEM high glucose for SILAC is a specially formulated cell culture medium designed to facilitate stable isotope labeling by amino acids in cell culture (SILAC) experiments. This medium is devoid of L-lysine and L-arginine, allowing researchers to incorporate isotopically labeled amino acids of their choice for precise quantitative proteomics. The high glucose concentration supports robust cell growth and metabolism, ensuring that cells proliferate effectively during the labeling process. By culturing cells in this high-glucose SILAC medium, researchers can achieve precise and reliable quantification of protein expression changes under conditions mimicking hyperglycemia or other high-glucose states, which is crucial for studying diseases like diabetes, cancer metabolism, etc. |
Synonyms | High glucose DMEM for SILAC; SILAC DMEM with high glucose; SILAC-compatible high glucose DMEM; High-glucose DMEM for stable isotope labeling |
Form | Liquid |
Storage | Store at 2-8°C for long term storage. |
Application | DMEM high glucose for SILAC is a specialized cell culture medium tailored for stable isotope labeling by amino acids in cell culture (SILAC) experiments, particularly when a high glucose environment is essential. By using this medium, scientists can achieve uniform and efficient incorporation of labeled amino acids into cellular proteins, enabling accurate mass spectrometry analysis for protein identification, quantification, and studies of protein dynamics in various biological processes. |