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Quantitative Human ADORA2a (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX352

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Specifications
Product Description
The quantitative human ADORA2a (glycosylated) sandwich ELISA kit is designed to detect human adenosine A2A receptor (ADORA2a) levels. ADORA2a is a G protein-coupled receptor, which binds to adenosine. ADORA2a plays a role in regulating blood flow. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.103 ng/mL, which can accurately detect low concentrations of ADORA2a in the sample.
Target
ADORA2a
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.103 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adenosine A2A receptor
Alternate Names
ADORA2a; Adenosine A2A receptor; ADORA2; hA2AR
Uniprot No.
N-Glycosylation Site
154
Application Notes
Application
The quantitative human ADORA2a (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ADORA2a levels. It is useful for studying the role of ADORA2a in vasodilation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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