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Quantitative Human CCR5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX454

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Specifications
Product Description
The quantitative human CCR5 (glycosylated) sandwich ELISA kit is designed to detect human chemokine C-C-motif receptor 5 (CCR5) levels. CCR5 is a G protein-coupled receptor that binds certain chemokines, which is expressed on immune cells, including T cells and macrophages. CCR5 plays a crucial role in the migration of these cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.152 ng/mL, which can accurately detect low concentrations of CCR5 in the sample.
Target
CCR5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.152 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Chemokine C-C-motif receptor 5
Alternate Names
CCR5; Chemokine C-C-motif receptor 5; CD195; CC-CKR5; CCCKR5; CMKBR5
Uniprot No.
O-Glycosylation Site
6, 7
Application Notes
Application
The quantitative human CCR5 (glycosylated) sandwich ELISA kit is designed to measure glycosylated CCR5 levels in samples. This measurement is valuable in research related to immunology and HIV infection. The kit is applied in studies of conditions involving chemokine signaling, which helps in understanding the role of CCR5.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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