GlycoFlux™ Lenti-Human SIGLEC5 Viral Particle

GlycoFlux™ Lenti-SIGLEC5 Viral Particle introduces SIGLEC5, a CD33-related inhibitory receptor on monocytes, macrophages, and neutrophils that also participates in host-pathogen interactions (e.g., with Group B Streptococcus). This platform is intended for stable mammalian line construction to examine inhibitory signaling circuits, immune evasion, and ligand recognition, with easy selection-based cloning or fluorescence sorting.

Recombinant Lentivirus

SIGLEC5

Human

EF1a

Lentivirus

HEK293T

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Sialic Acid Binding Ig Like Lectin 5

CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5; Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins have structural motifs in their immunoglobulin-like domains and sialic acid recognition sites in the first Ig V-set domain. The encoded protein, part of the CD33-related Siglec subgroup, inhibits activation in monocytes, macrophages, and neutrophils. It also plays a role in immune evasion by Group B Streptococcus (GBS).