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Immobilized pepsin (agarose resin) is prepared as an efficient digestion agent by immobilizing high-quality pepsin on beaded agarose. It can quickly digest different IgGs in complex samples, generate and purify F(ab')2 fragments, and has high stability and substrate specificity. It is easy to use and the reaction can be terminated by directly removing the agarose gel by centrifugation.
Immobilized pepsin (agarose resin) is widely used in protein structure and functions research and proteomics. It can specifically cleave the heavy chain of the hinge region of antibodies to generate and purify F(ab')2 fragments. In addition, it also has important uses in protease digestion and active peptide generation.