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Quantitative Human GPA33 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX770

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Specifications
Product Description
The quantitative human GPA33 (glycosylated) sandwich ELISA kit is designed to detect human glycoprotein A33 (GPA33) levels. GPA33 is a transmembrane protein that is expressed on epithelial cells. GPA33 plays a role in cell adhesion, which is involved in cell-to-cell interactions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.452 ng/mL, which can accurately detect low concentrations of GPA33 in the sample.
Target
GPA33
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.452 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2 ng/mL-40 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glycoprotein A33
Alternate Names
GPA33; Glycoprotein A33; Cell surface A33 antigen
Uniprot No.
N-Glycosylation Site
112, 200, 223
Application Notes
Application
The quantitative human GPA33 (glycosylated) sandwich ELISA kit is useful for measuring GPA33 levels in the samples. This measurement is relevant to research on cell adhesion and epithelial cells. The kit also has potential applications in investigations of cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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