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Quantitative Human GPA33 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX770)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GPA33 (glycosylated) sandwich ELISA kit is designed to detect human glycoprotein A33 (GPA33) levels. GPA33 is a transmembrane protein that is expressed on epithelial cells. GPA33 plays a role in cell adhesion, which is involved in cell-to-cell interactions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.452 ng/mL, which can accurately detect low concentrations of GPA33 in the sample.
Target GPA33
N-Glycosylation Site 112, 200, 223
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.452 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Glycoprotein A33
Alternate Names GPA33; Glycoprotein A33; Cell surface A33 antigen
Uniprot No. Q99795
Application The quantitative human GPA33 (glycosylated) sandwich ELISA kit is useful for measuring GPA33 levels in the samples. This measurement is relevant to research on cell adhesion and epithelial cells. The kit also has potential applications in investigations of cancer.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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