Glu-C Protease, Sequencing Grade (CAT#: GLJF-0225-HX22)

Product Description	Glu-C protease (sequencing grade) is a serine protease from Staphylococcus aureus V8. It is specific and cleaves at the carboxyl terminus of aspartic acid or glutamic acid residues. The enzyme exhibits optimal activity in the pH range of 4.0 to 9.0. Glu-C is suitable for protein digestion in solution, but is not recommended for in-gel digestion. It can be used alone or in combination with other proteases to obtain protein digests.
Synonyms	Glutamic acid-specific endopeptidase, sequencing grade; Glu-C endopeptidase, sequencing grade; Sequencing grade Glu-C protease; Glu-C protease for protein sequencing
MW	27 kDa
Source	Staphylococcus aureus V8
Form	Lyophilized powder
Storage	Store at 2-8°C for long term storage.

Application

Glu-C protease (sequencing grade) is a highly specific protease widely used in proteomics and biomedical research. It can specifically cleave at the carboxyl end of glutamic acid residues, and can also cleave aspartic acid residues under certain conditions. This enzymatic cleavage property makes Glu-C an important tool in protein mass spectrometry analysis, especially for structural analysis of complex proteins and identification of post-translational modification sites (such as phosphorylation and glycosylation). Using sequencing-grade Glu-C for protein digestion can obtain unique peptide sequence information, supplement the deficiencies of traditional trypsin digestion, and improve protein coverage and identification accuracy. This is of great significance for in-depth research on protein function, disease mechanisms, and the discovery of new drug targets.