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Quantitative Human IER3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX426

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Specifications
Product Description
The quantitative human IER3 (glycosylated) sandwich ELISA kit is designed to detect human immediate early response 3 (IER3) levels. IER3 is a protein that is rapidly induced in response to various cellular stresses and growth factors. IER3 can regulate cell growth, apoptosis, and differentiation. It plays a role in cellular responses to environmental changes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.105 ng/mL, which can accurately detect low concentrations of IER3 in the sample.
Target
IER3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.105 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Immediate early response 3
Alternate Names
IER3; Immediate early response 3; PRG1; DIF2; IEX-1L; Radiation-inducible immediate-early gene IEX-1
Uniprot No.
N-Glycosylation Site
133
Application Notes
Application
The quantitative human IER3 (glycosylated) sandwich ELISA kit is designed to measure glycosylated IER3 levels in samples. This measurement is valuable in research related to cellular stress responses and disease pathogenesis. The kit is applied in studies of conditions such as cancer and inflammation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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