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Quantitative Human LTβ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX532

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Specifications
Product Description
The quantitative human LTβ (glycosylated) sandwich ELISA kit is designed to detect human lymphotoxin β (LTβ) levels. LTβ is a protein that belongs to the tumor necrosis factor (TNF) superfamily, which plays a crucial role in the development and organization of lymphoid tissues, such as lymph nodes and spleen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.845 pg/mL, which can accurately detect low concentrations of LTβ in the sample.
Target
LTβ
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.845 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lymphotoxin β
Alternate Names
LTβ; Lymphotoxin β; LT-B; TNFC; TNFSF3; Tumor necrosis factor ligand superfamily member 3; Tumor necrosis factor C
Uniprot No.
N-Glycosylation Site
222
Application Notes
Application
The quantitative human LTβ (glycosylated) sandwich ELISA kit is a valuable tool for research in immunology and developmental biology, particularly in studies of lymphoid tissue development. Researchers use it to measure LTβ levels in tissue samples and cell culture supernatants. The kit has applications in studies of immune system development and inflammatory diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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