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Quantitative Human PCDH15 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX749)

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1×48 T
1×96 T

Product Description The quantitative human PCDH15 (glycosylated) sandwich ELISA kit is designed to detect human protocadherin 15 (PCDH15) levels. PCDH15 is a cell adhesion molecule that is a member of the cadherin superfamily. PCDH15 plays a role in the development of the inner ear. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.157 ng/mL, which can accurately detect low concentrations of PCDH15 in the sample.
Target PCDH15
N-Glycosylation Site 52, 97, 201, 419, 559, 662, 724, 768, 821, 851, 1064, 1084, 1175
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.157 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protocadherin 15
Alternate Names PCDH15; Protocadherin 15; CDHR15; Cadherin-related family member 15
Uniprot No. Q96QU1
Application The quantitative human PCDH15 (glycosylated) sandwich ELISA kit is useful for measuring PCDH15 levels in the samples. This measurement is relevant to research on hearing and inner ear development. The kit also has potential applications in investigations of hearing loss.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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