Quantitative Human PLA2G2D (Glycosylated) Sandwich ELISA Kit

The quantitative human PLA2G2D (glycosylated) sandwich ELISA kit is designed to detect human phospholipase A2, group 2D (PLA2G2D) levels. PLA2G2D is an enzyme that belongs to the phospholipase A2 superfamily. This enzyme catalyzes the hydrolysis of membrane phospholipids, releasing fatty acids like arachidonic acid. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.135 ng/mL, which can accurately detect low concentrations of PLA2G2D in the sample.

PLA2G2D

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.135 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Phospholipase A2, group 2D

PLA2G2D; Phospholipase A2, group 2D; sPLA2; SPLASH; Secreted phospholipase A2

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The quantitative human PLA2G2D (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PLA2G2D levels in biological samples. The assay can be utilized to investigate the role of PLA2G2D in various physiological and pathological conditions, including inflammatory diseases, and cellular signaling pathways.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.