GlycoErase™ ALDOA Knockout HEK293 Cell Line

GlycoErase™ ALDOA knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking ALDOA expression. ALDOA is a glycolytic enzyme that catalyzes the cleavage of fructose-1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Mutations in ALDOA are associated with Glycogen Storage Disease XII and linked to hemolytic anemia, while its dysregulation has been implicated in tumor progression. The knockout of ALDOA in the HEK293 background provides a human-relevant system for dissecting glycolytic control, metabolic flux, and cancer-associated energy reprogramming, and supports applications in metabolic disorder modeling, cancer biology, and glycolysis-targeted therapeutic development.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALDOA

Aldolase, Fructose-Bisphosphate A

ALDA; GSD12; HEL-S-87p

16p11.2

This class I fructose-bisphosphate aldolase catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Isozymes A, B, and C are expressed at different developmental stages. Mutations cause Glycogen Storage Disease XII and are associated with cancer. Pseudogenes are found on chromosomes 3 and 10.