GlycoErase™ ALDOA Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF378)

Description	GlycoErase™ ALDOA knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking ALDOA expression. ALDOA is a glycolytic enzyme that catalyzes the cleavage of fructose-1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Mutations in ALDOA are associated with Glycogen Storage Disease XII and linked to hemolytic anemia, while its dysregulation has been implicated in tumor progression. The knockout of ALDOA in the HEK293 background provides a human-relevant system for dissecting glycolytic control, metabolic flux, and cancer-associated energy reprogramming, and supports applications in metabolic disorder modeling, cancer biology, and glycolysis-targeted therapeutic development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALDOA
Full Name	Aldolase, Fructose-Bisphosphate A
Alternative Name	ALDA; GSD12; HEL-S-87p
Location	16p11.2
Gene ID	226
Summary	This class I fructose-bisphosphate aldolase catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Isozymes A, B, and C are expressed at different developmental stages. Mutations cause Glycogen Storage Disease XII and are associated with cancer. Pseudogenes are found on chromosomes 3 and 10.