GlycoErase™ ALDOA Knockout HepG2 Cell Line

GlycoErase™ ALDOA knockout HepG2 cell line removes ALDOA expression, a glycolytic enzyme catalyzing the cleavage of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. ALDOA mutations are linked to Glycogen Storage Disease XII and hemolytic anemia, and its dysregulation is associated with cancer progression. This knockout enables analysis of glycolytic flux, metabolic reprogramming, and energy regulation, supporting cancer metabolism and liver-specific metabolic studies.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALDOA

Aldolase, Fructose-Bisphosphate A

ALDA; GSD12; HEL-S-87p

16p11.2

This gene encodes a class I fructose-bisphosphate aldolase, a glycolytic enzyme that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, C) are differentially expressed during development. Mutations can lead to glycogen storage disease XII with hemolytic anemia and have been implicated in cancer progression. Related pseudogenes exist on chromosomes 3 and 10.