GlycoErase™ EPM2A Knockout HEK293 Cell Line

GlycoErase™ EPM2A knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which EPM2A expression has been deleted. EPM2A encodes a dual-specificity phosphatase that prevents glycogen hyperphosphorylation and insoluble aggregate formation. Mutations in EPM2A cause Lafora disease, a fatal neurodegenerative disorder characterized by glycogen inclusions. The knockout of EPM2A in the HEK293 background provides a human-based model to investigate glycogen regulation, Lafora body formation, and neurodegenerative mechanisms, supporting applications in glycogen metabolism research, rare disease modeling, and neurobiology.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

EPM2A

Epm2A Glucan Phosphatase, Laforin

EPM2; MELF; MELF2

6q24.3

The encoded dual-specificity phosphatase prevents glycogen hyperphosphorylation, avoiding insoluble aggregate formation. Loss-of-function mutations cause Lafora disease, a fatal, progressive myoclonus epilepsy characterized by Lafora bodies.