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GlycoFlux™ MSCV-Human PRKAA1 Viral Particle (CAT#: GLJF-1025-JF752)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MSCV-PRKAA1 Viral Particle drives AMPK α1 (PRKAA1) to model energy-stress signaling and metabolic enzyme phosphorylation, with MSCV supporting high-efficiency stable integration and persistent expression to establish robust mammalian lines.
Product Type Recombinant MSCV Retrovirus
Target PRKAA1
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Protein Kinase Amp-Activated Catalytic Subunit Alpha 1
Alternative Name AMPK; AMPKa1; AMPK alpha 1
Location 5p13.1
Gene ID 5562
Summary The encoded protein is the alpha catalytic chain of AMP-activated protein kinase (AMPK), which responds to increased AMP/ATP ratios by phosphorylating metabolic enzymes to conserve energy. Several isoforms arise from alternative transcripts.
For Research Use Only.
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