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Quantitative Human ADCY1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX540

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Specifications
Product Description
The quantitative human ADCY1 (glycosylated) sandwich ELISA kit is designed to detect human adenylate cyclase 1 (ADCY1) levels. ADCY1 is an enzyme that produces cyclic AMP (cAMP) from ATP. ADCY1 is predominantly expressed in the brain, which plays a significant role in synaptic plasticity, learning, and memory. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.492 ng/mL, which can accurately detect low concentrations of ADCY1 in the sample.
Target
ADCY1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.492 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.25 ng/mL-45 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adenylate cyclase 1
Alternate Names
ADCY1; Adenylate cyclase 1; AC1; ATP pyrophosphate-lyase 1; Adenylate cyclase type I
Uniprot No.
N-Glycosylation Site
704
Application Notes
Application
The quantitative human ADCY1 (glycosylated) sandwich ELISA kit is a valuable tool for research in neuroscience and pharmacology, particularly in studies of cAMP signaling in the brain. Researchers use it to measure ADCY1 levels in brain tissue. The kit has applications in studies of neurological disorders, learning, and memory.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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