Quantitative Human ARSF (Glycosylated) Sandwich ELISA Kit

The quantitative human ARSF (glycosylated) sandwich ELISA kit is designed to detect human arylsulfatase F (ARSF) levels. ARSF is an enzyme that catalyzes the hydrolysis of sulfate esters, which is located in the lysosomes. ARSF activity is involved in the degradation of certain sulfated molecules. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.126 ng/mL, which can accurately detect low concentrations of ARSF in the sample.

ARSF

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.126 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Arylsulfatase F

ARSF; Arylsulfatase F; ASF

50, 117, 336

The quantitative human ARSF (glycosylated) sandwich ELISA kit is designed to measure glycosylated ARSF levels in samples. This measurement is valuable in research related to lysosomal functions. The kit is applied in studies of metabolic diseases, which helps in understanding the role of ARSF.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.