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Quantitative Human CRLF1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX88

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Specifications
Product Description
The quantitative human CRLF1 (glycosylated) sandwich ELISA kit is designed to detect human cytokine receptor like factor 1 (CRLF1) levels. CRLF1 is a protein involved in regulating cytokine signaling and has been implicated in development and immune responses. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.110 ng/mL, which can accurately detect low concentrations of CRLF1 in the sample.
Target
CRLF1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.110 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cytokine receptor like factor 1
Alternate Names
CRLF1; Cytokine receptor like factor 1; CISS1; CLF; Cytokine-like factor 1
Uniprot No.
N-Glycosylation Site
92, 104, 140, 168, 292, 382
Application Notes
Application
The quantitative human CRLF1 (glycosylated) sandwich ELISA kit is valuable for research investigating CRLF1's role in hematopoiesis, immune regulation, and developmental disorders. The quantitative data obtained from this kit allows for precise analysis of CRLF1 levels, contributing to a better understanding of its involvement in these biological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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