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Quantitative Human ECP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX268)

Datasheet
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1×96 T
5×96 T
10×96 T

Product Description The quantitative human ECP (glycosylated) sandwich ELISA kit is designed to detect human eosinophil cationic protein (ECP) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.412 ng/mL, which can accurately detect low concentrations of ECP in the sample.
Target ECP
N-Glycosylation Site 84, 92, 119
Sample Types Tissue homogenates, serum, plasma
Sample Volume 50 μL-100 μL
Sensitivity 0.412 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.65 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Eosinophil cationic protein
Alternate Names Cytotoxic ribonuclease; Eosinophil cationic protein; Ribonuclease 3; Ribonuclease; RNase A family 3; RNase 3; RNASE3; RNS3; ECP
Uniprot No. P12724
Application The quantitative human ECP (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of ECP, helping to understand its role in conditions such as allergic reactions and asthma.
Kit Components Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated ECP antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-95%;
Plasma sample: n=4, 90-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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