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Quantitative Human EFNB1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX507)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EFNB1 (glycosylated) sandwich ELISA kit is designed to detect human ephrin B1 (EFNB1) levels. EFNB1 is a transmembrane protein that functions as a ligand for Eph receptors. Ephrin-B1 and Eph receptors are key components of the Eph/ephrin signaling pathway. This pathway is involved in a wide range of developmental processes, including axon guidance, cell migration, and tissue boundary formation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of EFNB1 in the sample.
Target EFNB1
N-Glycosylation Site 139
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.099 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ephrin B1
Alternate Names EFNB1; Ephrin B1; CFND; EPLG2; EPH-related receptor tyrosine kinase ligand 2
Uniprot No. P98172
Application The quantitative human EFNB1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EFNB1 levels. This kit is a valuable tool for investigating the role of EFNB1 and Eph/ephrin signaling in various biological processes and diseases. Researchers use it to measure EFNB1 levels in different experimental models. The kit has applications in developmental biology, neuroscience, and cancer research, where Eph/ephrin signaling is often dysregulated.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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