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Quantitative Human EFNB1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX507

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Specifications
Product Description
The quantitative human EFNB1 (glycosylated) sandwich ELISA kit is designed to detect human ephrin B1 (EFNB1) levels. EFNB1 is a transmembrane protein that functions as a ligand for Eph receptors. Ephrin-B1 and Eph receptors are key components of the Eph/ephrin signaling pathway. This pathway is involved in a wide range of developmental processes, including axon guidance, cell migration, and tissue boundary formation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of EFNB1 in the sample.
Target
EFNB1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.099 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin B1
Alternate Names
EFNB1; Ephrin B1; CFND; EPLG2; EPH-related receptor tyrosine kinase ligand 2
Uniprot No.
N-Glycosylation Site
139
Application Notes
Application
The quantitative human EFNB1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EFNB1 levels. This kit is a valuable tool for investigating the role of EFNB1 and Eph/ephrin signaling in various biological processes and diseases. Researchers use it to measure EFNB1 levels in different experimental models. The kit has applications in developmental biology, neuroscience, and cancer research, where Eph/ephrin signaling is often dysregulated.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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