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Quantitative Human FFAR4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX629

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Specifications
Product Description
The quantitative human FFAR4 (glycosylated) sandwich ELISA kit is designed to detect human free fatty acid receptor 4 (FFAR4) levels. FFAR4 is a G protein-coupled receptor that is activated by long-chain omega-3 fatty acids. This receptor plays a role in regulating inflammation, glucose homeostasis, and lipid metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.525 ng/mL, which can accurately detect low concentrations of FFAR4 in the sample.
Target
FFAR4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.525 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2 ng/mL-40 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Free fatty acid receptor 4
Alternate Names
FFAR4; Free fatty acid receptor 4; PGR4; GPR120; O3FAR1; G protein coupled receptor 120; Omega-3 fatty acid receptor 1
Uniprot No.
N-Glycosylation Site
21
Application Notes
Application
The quantitative human FFAR4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated FFAR4 levels in biological samples. The kit is applicable in studies of metabolic disorders, inflammation, and fatty acid signaling. It is useful in fields such as endocrinology and nutrition.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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