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Quantitative Human GRIA1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX409

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Specifications
Product Description
The quantitative human GRIA1 (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, AMPA1 (GRIA1) levels. GRIA1 is a subunit of the AMPA receptor. AMPA receptors are ligand-gated ion channels in the central nervous system. GRIA1 plays a crucial role in fast synaptic transmission, which is fundamental for learning, memory, and neuronal communication. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.058 ng/mL, which can accurately detect low concentrations of GRIA1 in the sample.
Target
GRIA1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.058 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, AMPA1
Alternate Names
GRIA1; Glutamate receptor, ionotropic, AMPA1; GLUH1; AMPA-selective glutamate receptor 1
Uniprot No.
N-Glycosylation Site
63, 249, 257, 363, 401, 406
Application Notes
Application
The quantitative human GRIA1 (glycosylated) sandwich ELISA kit is used to measure glycosylated GRIA1 levels in samples. This measurement is valuable in neuroscience research, particularly in studies of synaptic functions and neurological disorders. The kit is applied in investigations of conditions such as Alzheimer's disease, epilepsy, and stroke, where GRIA1 expression or functions may be altered.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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