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Quantitative Human HS6ST1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX67)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human HS6ST1 (glycosylated) sandwich ELISA kit is designed to detect human heparan sulfate-6-O-sulfotransferase 1 (HS6ST1) levels. HS6ST1 is an enzyme that catalyzes the 6-O-sulfation of heparan sulfate, a crucial modification that influences the interactions of heparan sulfate with various proteins and growth factors. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of HS6ST1 in the sample.
Target HS6ST1
N-Glycosylation Site 264, 320
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.099 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Heparan sulfate-6-O-sulfotransferase 1
Alternate Names HS6ST1; Heparan sulfate-6-O-sulfotransferase 1
Uniprot No. O60243
Application The quantitative human HS6ST1 (glycosylated) sandwich ELISA kit is essential for research exploring the role of HS6ST1 in processes like cell signaling, angiogenesis, and development, where heparan sulfate modifications are critical. The kit's quantitative data is valuable for understanding HS6ST1's involvement in both normal physiology and diseases, providing insights into its potential as a therapeutic target or biomarker.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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