Quantitative Human HS6ST1 (Glycosylated) Sandwich ELISA Kit

The quantitative human HS6ST1 (glycosylated) sandwich ELISA kit is designed to detect human heparan sulfate-6-O-sulfotransferase 1 (HS6ST1) levels. HS6ST1 is an enzyme that catalyzes the 6-O-sulfation of heparan sulfate, a crucial modification that influences the interactions of heparan sulfate with various proteins and growth factors. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of HS6ST1 in the sample.

HS6ST1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.099 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Heparan sulfate-6-O-sulfotransferase 1

HS6ST1; Heparan sulfate-6-O-sulfotransferase 1

264, 320

The quantitative human HS6ST1 (glycosylated) sandwich ELISA kit is essential for research exploring the role of HS6ST1 in processes like cell signaling, angiogenesis, and development, where heparan sulfate modifications are critical. The kit's quantitative data is valuable for understanding HS6ST1's involvement in both normal physiology and diseases, providing insights into its potential as a therapeutic target or biomarker.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.