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Quantitative Human ISR (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX183)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ISR (glycosylated) sandwich ELISA kit is designed to detect human insulin receptor (ISR) levels. ISR is a transmembrane receptor tyrosine kinase that mediates the biological effects of insulin, regulating glucose metabolism, cell growth, and differentiation. It is crucial for maintaining glucose homeostasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.792 ng/mL, which can accurately detect low concentrations of ISR in the sample.
Target ISR
N-Glycosylation Site 43, 52, 105, 138, 242, 282, 322, 364, 424, 445, 541, 633, 651, 698, 769, 782, 920, 933
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.792 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.5 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Insulin receptor
Alternate Names ISR; Insulin receptor; CD220; INSR; Insulin receptor subunit β
Uniprot No. P06213
Application The quantitative human ISR (glycosylated) sandwich ELISA kit is valuable in research investigating insulin resistance, diabetes, and related metabolic disorders. Researchers employ this ELISA to quantify ISR levels, providing insights into receptor expression and potential therapeutic targets.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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