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Quantitative Human MAN1A1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX376

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Specifications
Product Description
The quantitative human MAN1A1 (glycosylated) sandwich ELISA kit is designed to detect human mannosidase α class 1A member 1 (MAN1A1) levels. MAN1A1 is an enzyme that is located in the Golgi apparatus. MAN1A1 plays a role in the processing of N-linked glycans. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of MAN1A1 in the sample.
Target
MAN1A1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.095 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Mannosidase α class 1A member 1
Alternate Names
MAN1A1; Mannosidase α class 1A member 1; MAN9; Mannosidase alpha class 1A member 1
Uniprot No.
N-Glycosylation Site
513
Application Notes
Application
The quantitative human MAN1A1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated MAN1A1 levels. It is useful for studying the role of MAN1A1 in the Golgi apparatus.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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