Quantitative Human NAAA (Glycosylated) Sandwich ELISA Kit

The quantitative human NAAA (glycosylated) sandwich ELISA kit is designed to detect human N-Acylethanolamine acid amidase (NAAA) levels. NAAA is an enzyme that belongs to the acid ceramidase family, which catalyzes the hydrolysis of N-acyl amino acids, a class of lipid signaling molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of NAAA in the sample.

NAAA

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.140 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

N-Acylethanolamine acid amidase

NAAA; N-Acylethanolamine acid amidase; ASAHL; Acid ceramidase-like protein

37, 107, 309, 333

The quantitative human NAAA (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry, pharmacology, and neuroscience, particularly in studies of lipid signaling and inflammation. Researchers use it to measure NAAA levels in tissue samples and cell extracts. The kit has applications in studies of pain, inflammation, and neurodegenerative diseases.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.