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Quantitative Human PAR1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX330)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PAR1 (glycosylated) sandwich ELISA kit is designed to detect human protease activated receptor 1 (PAR1) levels. PAR1 is a G protein-coupled receptor. It is activated by thrombin. PAR1 plays a role in blood clotting. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.061 ng/mL, which can accurately detect low concentrations of PAR1 in the sample.
Target PAR1
N-Glycosylation Site 35, 62, 75, 250, 259
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.061 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protease activated receptor 1
Alternate Names PAR1; Protease activated receptor 1; CF2R; HTR; Coagulation factor II receptor
Uniprot No. P25116
Application The quantitative human PAR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PAR1 levels. It is useful for studying the role of PAR1 in platelet activation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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