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Quantitative Human PAR1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX330

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Specifications
Product Description
The quantitative human PAR1 (glycosylated) sandwich ELISA kit is designed to detect human protease activated receptor 1 (PAR1) levels. PAR1 is a G protein-coupled receptor. It is activated by thrombin. PAR1 plays a role in blood clotting. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.061 ng/mL, which can accurately detect low concentrations of PAR1 in the sample.
Target
PAR1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.061 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protease activated receptor 1
Alternate Names
PAR1; Protease activated receptor 1; CF2R; HTR; Coagulation factor II receptor
Uniprot No.
N-Glycosylation Site
35, 62, 75, 250, 259
Application Notes
Application
The quantitative human PAR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PAR1 levels. It is useful for studying the role of PAR1 in platelet activation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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