Quantitative Human PDGFA (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX163)

Product Description	The quantitative human PDGFA (glycosylated) sandwich ELISA kit is designed to detect human platelet derived growth factor subunit A (PDGFA) levels. PDGFA is a growth factor that plays a crucial role in cell proliferation, differentiation, and migration, contributing to processes like angiogenesis and wound healing. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of PDGFA in the sample.
Target	PDGFA
N-Glycosylation Site	134
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.098 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Platelet derived growth factor subunit A
Alternate Names	PDGFA; Platelet derived growth factor subunit A; PDGF1; Platelet derived growth factor alpha polypeptide
Uniprot No.	P04085

Application	The quantitative human PDGFA (glycosylated) sandwich ELISA kit is utilized in research investigating diseases such as cancer, atherosclerosis, and fibrosis, where PDGFA signaling is implicated. Researchers employ this ELISA to quantify PDGFA levels, providing insights into its role in these conditions and potential therapeutic targeting.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 95-110%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.