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Quantitative Human PDGFA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX163

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Specifications
Product Description
The quantitative human PDGFA (glycosylated) sandwich ELISA kit is designed to detect human platelet derived growth factor subunit A (PDGFA) levels. PDGFA is a growth factor that plays a crucial role in cell proliferation, differentiation, and migration, contributing to processes like angiogenesis and wound healing. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of PDGFA in the sample.
Target
PDGFA
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.098 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Platelet derived growth factor subunit A
Alternate Names
PDGFA; Platelet derived growth factor subunit A; PDGF1; Platelet derived growth factor alpha polypeptide
Uniprot No.
N-Glycosylation Site
134
Application Notes
Application
The quantitative human PDGFA (glycosylated) sandwich ELISA kit is utilized in research investigating diseases such as cancer, atherosclerosis, and fibrosis, where PDGFA signaling is implicated. Researchers employ this ELISA to quantify PDGFA levels, providing insights into its role in these conditions and potential therapeutic targeting.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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