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Quantitative Human PNPLA3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX851

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Specifications
Product Description
The quantitative human PNPLA3 (glycosylated) sandwich ELISA kit is designed to detect human patatin like phospholipase domain containing protein 3 (PNPLA3) levels. PNPLA3 is an enzyme that has lipase and acylglycerol lipase activity. PNPLA3 is involved in lipid metabolism. This enzyme plays a role in the breakdown of triglycerides. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of PNPLA3 in the sample.
Target
PNPLA3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.093 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Patatin like phospholipase domain containing protein 3
Alternate Names
PNPLA3; Patatin like phospholipase domain containing protein 3; ADPN; C22orf20; Adiponutrin
Uniprot No.
N-Glycosylation Site
89, 280
Application Notes
Application
The quantitative human PNPLA3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of PNPLA3 in biological samples. This kit is applicable in studies examining lipid metabolism and fatty liver disease. It is also relevant to studies investigating the role of PNPLA3 genetic variations in influencing liver fat content and disease risk.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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