GlycoErase™ PHKA2 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF668)

Description	GlycoErase™ PHKA2 knockout HepG2 cell line removes PHKA2 expression, encoding the hepatic isoform of the phosphorylase kinase alpha subunit. Mutations cause glycogen storage disease type IXA. This knockout is ideal for modeling liver-specific glycogen metabolism, energy regulation, and metabolic liver disease.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKA2
Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name	PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location	Xp22.13
Gene ID	5256
Summary	Phosphorylase kinase is assembled from 16 total subunits-four each of alpha, beta, gamma, and delta types. In this form, the hepatic alpha isoform is produced by this gene, while the gamma unit hosts the catalytic site. The delta subunit is a calmodulin that manages calcium dependence. Variants in this gene cause glycogen storage disease type 9A. Several splice forms have been detected but remain incompletely characterized.