GlycoFlux™ Human PHKA2 Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF436)

Description	GlycoFlux™ human PHKA2 overexpression HEK293 cell line boosts PHKA2, the hepatic α-subunit of phosphorylase kinase. Defects lead to glycogen storage disease IXa, and this engineered system provides a model for liver glycogen metabolism and X-linked glycogenosis.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKA2
Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name	PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location	Xp22.13
Gene ID	5256
Summary	Phosphorylase kinase is a 16-subunit enzyme composed of four alpha, beta, gamma, and delta subunits. The alpha subunit includes skeletal muscle and hepatic isoforms, with the hepatic form encoded by this gene. The gamma subunit holds the active site, while alpha and beta are regulatory. The delta subunit, a calmodulin, mediates calcium dependence. Mutations cause glycogen storage disease type 9A (X-linked liver glycogenosis). Alternative splice variants have been reported, though full-length forms are not defined.