Quantitative Human 5HTR4 (Glycosylated) Sandwich ELISA Kit

The quantitative human 5HTR4 (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 4 (5HTR4) levels. 5HTR4 is a G protein-coupled receptor for serotonin. Serotonin is a neurotransmitter that affects mood, appetite, and gastrointestinal motility. 5HTR4 is expressed in the brain and gut, which modulates neuronal activity and smooth muscle contraction. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of 5HTR4 in the sample.

5HTR4

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.091 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

5-Hydroxytryptamine receptor 4

5HTR4; 5-Hydroxytryptamine receptor 4; 5-HT4; 5-HT4R

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The quantitative human 5HTR4 (glycosylated) sandwich ELISA kit is a valuable tool for research in neuroscience and gastroenterology, particularly in studies of serotonin signaling. Researchers can measure 5HTR4 levels in tissues. Applications include studies of depression, anxiety, and gastrointestinal disorders.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.