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Quantitative Human BCHE (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX184)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human BCHE (glycosylated) sandwich ELISA kit is designed to detect human butyrylcholinesterase (BCHE) levels. BCHE is a plasma enzyme that hydrolyzes choline esters, including succinylcholine and certain local anesthetics, playing a role in drug metabolism and detoxification. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of BCHE in the sample.
Target BCHE
N-Glycosylation Site 45, 85, 134, 269, 284, 369, 483, 509, 513, 514
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.129 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Butyrylcholinesterase
Alternate Names BCHE; Butyrylcholinesterase; BuChE; CHE1; Pseudocholinesterase; Cholinesterase
Uniprot No. P06276
Application The quantitative human BCHE (glycosylated) sandwich ELISA kit is valuable in research investigating drug metabolism, pharmacogenetics, and liver function. Researchers utilize this ELISA to quantify BCHE levels, providing insights into individual variations in drug response and potential diagnostic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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