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Quantitative Human BCHE (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX184

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Specifications
Product Description
The quantitative human BCHE (glycosylated) sandwich ELISA kit is designed to detect human butyrylcholinesterase (BCHE) levels. BCHE is a plasma enzyme that hydrolyzes choline esters, including succinylcholine and certain local anesthetics, playing a role in drug metabolism and detoxification. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of BCHE in the sample.
Target
BCHE
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.129 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Butyrylcholinesterase
Alternate Names
BCHE; Butyrylcholinesterase; BuChE; CHE1; Pseudocholinesterase; Cholinesterase
Uniprot No.
N-Glycosylation Site
45, 85, 134, 269, 284, 369, 483, 509, 513, 514
Application Notes
Application
The quantitative human BCHE (glycosylated) sandwich ELISA kit is valuable in research investigating drug metabolism, pharmacogenetics, and liver function. Researchers utilize this ELISA to quantify BCHE levels, providing insights into individual variations in drug response and potential diagnostic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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