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Quantitative Human COCH (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX54

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Specifications
Product Description
The quantitative human COCH (glycosylated) sandwich ELISA kit is designed to detect human cochlin (COCH) levels. Cochlin is an inner ear protein encoded by the COCH gene, essential for maintaining the structure and function of the inner ear's extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.121 ng/mL, which can accurately detect low concentrations of COCH in the sample.
Target
COCH
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.121 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cochlin
Alternate Names
COCH; Cochlin; COCH5B2; DFNA31; Coagulation factor C
Uniprot No.
N-Glycosylation Site
100, 221
Application Notes
Application
The quantitative human COCH (glycosylated) sandwich ELISA kit is used in research to investigate the expression patterns of Cochlin, particularly in studies related to inner ear diseases, hearing impairment, and vestibular dysfunction. By quantifying Cochlin levels, researchers can explore its role in the pathogenesis of auditory and balance disorders and evaluate the potential impact of therapeutic interventions targeting this protein.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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