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Quantitative Human COL3α1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX151)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL3α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 3 α1 (COL3α1) levels. COL3α1 is a fibrillar collagen that plays a critical role in the structural integrity of extensible connective tissues, such as skin, blood vessels, and internal organs. It is essential for tissue elasticity and wound healing. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.250 ng/mL, which can accurately detect low concentrations of COL3α1 in the sample.
Target COL3α1
O-Glycosylation Site 263
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.250 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 3 α1
Alternate Names COL3α1; Collagen type 3 α1; COL3-A1; EDS4A; Collagen type III α1
Uniprot No. P02461
Application The quantitative human COL3α1 (glycosylated) sandwich ELISA kit is utilized in research investigating fibrosis, vascular diseases, and connective tissue disorders like Ehlers-Danlos syndrome. Researchers use this ELISA to quantify COL3α1 levels, providing insights into its role in tissue remodeling and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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