Quantitative Human COL3α1 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL3α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 3 α1 (COL3α1) levels. COL3α1 is a fibrillar collagen that plays a critical role in the structural integrity of extensible connective tissues, such as skin, blood vessels, and internal organs. It is essential for tissue elasticity and wound healing. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.250 ng/mL, which can accurately detect low concentrations of COL3α1 in the sample.

COL3α1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.250 ng/mL

Quantitative sandwich ELISA

2 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 3 α1

COL3α1; Collagen type 3 α1; COL3-A1; EDS4A; Collagen type III α1

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The quantitative human COL3α1 (glycosylated) sandwich ELISA kit is utilized in research investigating fibrosis, vascular diseases, and connective tissue disorders like Ehlers-Danlos syndrome. Researchers use this ELISA to quantify COL3α1 levels, providing insights into its role in tissue remodeling and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.