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Quantitative Human CRT (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX342)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CRT (glycosylated) sandwich ELISA kit is designed to detect human calreticulin (CRT) levels. CRT is a calcium-binding protein, which is located in the endoplasmic reticulum. CRT plays a role in protein folding. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CRT in the sample.
Target CRT
N-Glycosylation Site 344
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Calreticulin
Alternate Names CRT; Calreticulin; SLC6A8; CT1; CRTR; Solute carrier family 6 member 8
Uniprot No. P27797
Application The quantitative human CRT (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CRT levels. It is useful for studying the role of CRT in protein quality control.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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