Quantitative Human CRT (Glycosylated) Sandwich ELISA Kit

The quantitative human CRT (glycosylated) sandwich ELISA kit is designed to detect human calreticulin (CRT) levels. CRT is a calcium-binding protein, which is located in the endoplasmic reticulum. CRT plays a role in protein folding. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CRT in the sample.

CRT

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Calreticulin

CRT; Calreticulin; SLC6A8; CT1; CRTR; Solute carrier family 6 member 8

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The quantitative human CRT (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CRT levels. It is useful for studying the role of CRT in protein quality control.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.