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Quantitative Human ELA3B (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX208)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ELA3B (glycosylated) sandwich ELISA kit is designed to detect human elastase 3B (ELA3B) levels. ELA3B is a serine protease involved in the digestion of proteins, particularly elastin, and is primarily secreted by the pancreas. It plays a role in various physiological processes, including nutrient digestion and tissue remodeling. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.147 ng/mL, which can accurately detect low concentrations of ELA3B in the sample.
Target ELA3B
N-Glycosylation Site 114
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.147 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Elastase 3B
Alternate Names ELA3B; Elastase 3B; CELA3B; Chymotrypsin like elastase family member 3B; Protease E
Uniprot No. P08861
Application The quantitative human ELA3B (glycosylated) sandwich ELISA kit is valuable in research investigating pancreatic function, pancreatitis, and other digestive disorders. Researchers utilize this ELISA to quantify ELA3B levels, providing insights into pancreatic enzyme activity and potential diagnostic or prognostic applications related to digestive health.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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