Quantitative Human HSP47 (Glycosylated) Sandwich ELISA Kit

The quantitative human HSP47 (glycosylated) sandwich ELISA kit is designed to detect human heat shock protein 47 (HSP47) levels. HSP47 is a collagen-specific chaperone protein, which plays a crucial role in the folding and assembly of collagen molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 52.583 pg/mL, which can accurately detect low concentrations of HSP47 in the sample.

HSP47

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

52.583 pg/mL

Quantitative sandwich ELISA

150 pg/mL-6000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Heat shock protein 47

HSP47; Heat shock protein 47; CBP2; CBP1; Colligen; Collagen binding protein 2

120, 125

The quantitative human HSP47 (glycosylated) sandwich ELISA kit is designed to measure glycosylated HSP47 levels in samples. This measurement is valuable in research related to collagen biology. The kit is applied in studies of conditions affecting connective tissues, which helps in understanding the role of HSP47.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.