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Quantitative Human HSP47 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX444)

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1×48 T
1×96 T

Product Description The quantitative human HSP47 (glycosylated) sandwich ELISA kit is designed to detect human heat shock protein 47 (HSP47) levels. HSP47 is a collagen-specific chaperone protein, which plays a crucial role in the folding and assembly of collagen molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 52.583 pg/mL, which can accurately detect low concentrations of HSP47 in the sample.
Target HSP47
N-Glycosylation Site 120, 125
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 52.583 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 150 pg/mL-6000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Heat shock protein 47
Alternate Names HSP47; Heat shock protein 47; CBP2; CBP1; Colligen; Collagen binding protein 2
Uniprot No. P50454
Application The quantitative human HSP47 (glycosylated) sandwich ELISA kit is designed to measure glycosylated HSP47 levels in samples. This measurement is valuable in research related to collagen biology. The kit is applied in studies of conditions affecting connective tissues, which helps in understanding the role of HSP47.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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