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Quantitative Human JAM2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX481

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Specifications
Product Description
The quantitative human JAM2 (glycosylated) sandwich ELISA kit is designed to detect human junctional adhesion molecule 2 (JAM2) levels. JAM2 is a protein that belongs to the immunoglobulin superfamily, which is expressed at intercellular junctions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 13.533 pg/mL, which can accurately detect low concentrations of JAM2 in the sample.
Target
JAM2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
13.533 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Junctional adhesion molecule 2
Alternate Names
JAM2; Junctional adhesion molecule 2; CD322; JAMB; VEJAM; Junctional adhesion molecule B; Vascular endothelial junction-associated molecule
Uniprot No.
N-Glycosylation Site
98, 187, 236
Application Notes
Application
The quantitative human JAM2 (glycosylated) sandwich ELISA kit is designed to measure glycosylated JAM2 levels in samples. This measurement is valuable in research related to cell adhesion. The kit is applied in studies of vascular biology, which helps in understanding the role of JAM2.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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