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Quantitative Human KRT7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX207

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Specifications
Product Description
The quantitative human KRT7 (glycosylated) sandwich ELISA kit is designed to detect human keratin 7 (KRT7) levels. KRT7 is an intermediate filament protein expressed in simple and glandular epithelial tissues, playing a role in maintaining cellular structure and integrity. It is often used as a marker for certain types of carcinomas. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 29.992 pg/mL, which can accurately detect low concentrations of KRT7 in the sample.
Target
KRT7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
29.992 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Keratin 7
Alternate Names
KRT7; Keratin 7; CK7; Cytokeratin 7
Uniprot No.
O-Glycosylation Site
12
Application Notes
Application
The quantitative human KRT7 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer diagnostics, particularly for identifying and characterizing specific epithelial cancers, as well as in studies of epithelial tissue differentiation and function. Researchers utilize this ELISA to quantify KRT7 levels, providing insights into tumor classification and potential diagnostic or prognostic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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