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Quantitative Human LPCAT4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX634

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Specifications
Product Description
The quantitative human LPCAT4 (glycosylated) sandwich ELISA kit is designed to detect human lysophosphatidylcholine acyltransferase 4 (LPCAT4) levels. LPCAT4 is an enzyme that catalyzes the acylation of lysophosphatidylcholine (LPC) to form phosphatidylcholine (PC). This enzymatic activity is important in the metabolism of phospholipids. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.594 ng/mL, which can accurately detect low concentrations of LPCAT4 in the sample.
Target
LPCAT4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.594 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.25 ng/mL-45 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lysophosphatidylcholine acyltransferase 4
Alternate Names
LPCAT4; Lysophosphatidylcholine acyltransferase 4; AYTL3; LPEAT2; Acyltransferase like 3
Uniprot No.
N-Glycosylation Site
152
Application Notes
Application
The quantitative human LPCAT4 (glycosylated) sandwich ELISA kit provides a quantitative measurement of glycosylated LPCAT4 levels in biological samples. It is applicable in studies related to lipid metabolism, membrane biology, and phospholipid synthesis. The kit is valuable in fields such as biochemistry and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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