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Quantitative Human MUC5AC (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX790

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human MUC5AC (glycosylated) sandwich ELISA kit is designed to detect human mucin 5AC (MUC5AC) levels. It is suitable for various biological samples such as cell culture supernates, serum, plasma, or urine. Its sensitivity is 4.052 ng/mL, which can accurately detect low concentrations of MUC5AC in the sample.
Target
MUC5AC
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated MUC5AC antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Cell culture supernates, serum, plasma, urine
Sample Volume
50 μL-100 μL
Sensitivity
4.052 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
16 ng/mL-1000 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Mucin 5AC
Alternate Names
Mucin 5AC; Major airway glycoprotein; Tracheobronchial mucin; TBM; MUC5AC; MUC5; Mucin-5AC
Uniprot No.
P98088
N-Glycosylation Site
205, 258, 415, 524, 1308, 3774, 4869, 4942, 5057, 5093, 5236, 5347, 5377, 5386, 5455, 5528, 5591
O-Glycosylation Site
2395, 2405, 2451, 2461, 2531, 2541, 2571, 2581, 2699, 2709, 2883, 2893, 2979, 2989, 3067, 3077, 4224, 4234, 4296, 4306, 4320, 4330, 4376, 4386, 4440, 4450, 4480, 4490, 4512, 4522, 4568, 4578
C-Glycosylation Site
1389, 1584, 1749, 1957, 2122, 3228, 3526, 3959, 4633
Application Notes
Application
The quantitative human MUC5AC (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of MUC5AC, helping to understand its role in signal transduction.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 90-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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