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Quantitative Human NPHN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX72)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NPHN (glycosylated) sandwich ELISA kit is designed to detect human nephrin (NPHN) levels. NPHN is a crucial structural protein located in the glomerular slit diaphragm of kidney podocytes, playing a vital role in the kidney's filtration barrier. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, urine. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of NPHN in the sample.
Target NPHN
N-Glycosylation Site 40, 356, 401, 547, 553, 564, 577, 680, 708, 908
Sample Types Tissue homogenates, cell lysates, urine
Sample Volume 100 μL
Sensitivity 0.098 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Nephrin
Alternate Names NPHN; Nephrin; NPHS1; Nephrosis 1
Uniprot No. O60500
Application The quantitative human NPHN (glycosylated) sandwich ELISA kit is designed to measure the concentration of glycosylated Nephrin in biological samples, particularly urine and serum. This application is crucial for research and clinical diagnostics related to kidney diseases, such as nephrotic syndrome and diabetic nephropathy. The quantitative data provides valuable insights into the integrity of the glomerular filtration barrier and can be used to monitor disease progression and treatment efficacy.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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