Quantitative Human NPHN (Glycosylated) Sandwich ELISA Kit

The quantitative human NPHN (glycosylated) sandwich ELISA kit is designed to detect human nephrin (NPHN) levels. NPHN is a crucial structural protein located in the glomerular slit diaphragm of kidney podocytes, playing a vital role in the kidney's filtration barrier. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, urine. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of NPHN in the sample.

NPHN

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, urine

100 μL

0.098 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Nephrin

NPHN; Nephrin; NPHS1; Nephrosis 1

40, 356, 401, 547, 553, 564, 577, 680, 708, 908

The quantitative human NPHN (glycosylated) sandwich ELISA kit is designed to measure the concentration of glycosylated Nephrin in biological samples, particularly urine and serum. This application is crucial for research and clinical diagnostics related to kidney diseases, such as nephrotic syndrome and diabetic nephropathy. The quantitative data provides valuable insights into the integrity of the glomerular filtration barrier and can be used to monitor disease progression and treatment efficacy.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.