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Quantitative Human P2RY1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX429

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Specifications
Product Description
The quantitative human P2RY1 (glycosylated) sandwich ELISA kit is designed to detect human purinergic receptor P2Y, G protein coupled 1 (P2RY1) levels. P2RY1 is a G protein-coupled receptor that binds ATP and ADP. These nucleotides functions as signaling molecules. P2RY1 is expressed on various cell types, including platelets and neurons. Its activation is involved in platelet aggregation and neuronal communication. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of P2RY1 in the sample.
Target
P2RY1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.095 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Purinergic receptor P2Y, G protein coupled 1
Alternate Names
P2RY1; Purinergic receptor P2Y, G protein coupled 1; P2Y1; P2Y purinoceptor 1; ATP receptor
Uniprot No.
N-Glycosylation Site
11, 27, 113, 197
Application Notes
Application
The quantitative human P2RY1 (glycosylated) sandwich ELISA kit is used to measure glycosylated P2RY1 levels in samples. This measurement is important in research related to platelet functions and neuronal signaling. The kit is applied in studies of conditions such as thrombosis and neurological diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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